About me π
I love solving scientific problems, and I believe there is always a beautiful solution for everything. Even though I have a strong background in biochemistry, molecular biology, cell biology, and data analysis for biological research, I am always fascinated by multidisciplinary research, which emerged as an indispensable part of science. Life is more than just what we do in our area. Opening our vision to other fields will help us explore our true potential and produce innovative ideas.
Career π
July 2022 - Present
November 2021 - Present
- π MBA candidate, class of 2023, Quantic School of Business and Technology
2021 - July 2022
- Postdoc at Signal transduction laboratory, POSTECH Biotech Center
- Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea
2016 - 2021
- π Doctor of Philosophy in Life Science, Signal transduction laboratory
- Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea
2008 - 2013
- π Bachelor of Science in Biology, Stem cells laboratory
- University of Science, Saigon, Vietnam
Awards π
2021
- The zenith award for academic excellence from Quantic School of Business and Technology
2019
- βBest research awardβ from Korean Society for Biochemistry and Molecular Biology (KSBMB)
Skills πͺ
Wet-lab techniques π§ͺ
- Gene cloning, gene editing, gene knock down, knock out, knock in using cutting edge CRISPR/Cas technology and conventional way using RNA interference
- Protein/DNA/RNA purification using FPLC and analysis
- Cells sorting, cell analysis using FACS and advance cell culture techniques
- Immuno-staining techniques and advance image analysis from immunohistochemistry (IHC), immunofluorescent (IF), immunocytochemistry (ICC)
- Frequently used in vivo techniques, behavior tests, and surgery
Advance imaging techniques π¬
- Setting up of TIRF microscope system for live/fix cell imaging and super resolution imaging (dSTORM, PALM)
- Single molecule analysis of dynamic biomolecule using single particle tracking assay (SPT), prolong single particle tracking (Prolong SPT), co-immuno-immobilization assay (CoII)
- Data analysis using Matlab, Python for image processing, data pre-processing, bioinformatic, and statistic analysis
- Other popular software and toolkit for scientific research: GraphPad Prism, ImageJ, MIB for electron microscopy analysis
Publications π
2021
- Control of EGFR activation by inner leaflet cholesterol, Do-Hyeon Kim*, Hong Minh Triet*, Eduard Bocharov*, Eui Kim, Soyeon Park, Pavel Volynsky, Roman G. Efremov, Minkyu Jung, Kai Zhou, Yonghoon kwon, Yeonho Chang, Sung Ho Ryu, manuscript under preparation
- An Oxygen-independent Super-photostable Organic Dye for Long-term Single Protein Imaging in Live Cells, Do-Hyeon Kim*, Hong Minh Triet*, Sun Hyeok Lee, Soyeon Park, Young-Tae Chang, Sung Ho Ryu, manuscript under preparation
- Regulation of EGFR Activation and Signaling by Lipids on the plasma membrane, Do-Hyeon Kim*, Hong Minh Triet*, Sung Ho Ryu, Progress in Lipid Research, 6;83:101115
- Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging, Do-Hyeon Kim*, Yeonho Chang*, Soyeon Park, Min Gyu Jeong, Yonghoon Kwon, Kai Zhou, Jungeun Noh, Yun-Kyu Choi, Hong Minh Triet, Young-Tae Chang, Sung Ho Ryu, Chemical Science, 12(25):8660-8667
- Improved resolution in single-molecule localization microscopy using QD-PAINT, Yeonho Chang, Do-Hyeon Kim, Kai Zhou, Min Gyu Jeong, Soyeon Park, Yonghoon Kwon, Hong Minh Triet, Jungeun Noh, Sung Ho Ryu, Nature Experimental & Molecular Medicine, 53(3):384-392.
- These authors equally contributed to this work.